Piperlongumine Inhibits Migration of Glioblastoma Cells via Activation of ROS-Dependent p38 and JNK Signaling Pathways
Figure 4
Activation of p38 and JNK by ROS accumulation contributes to PL’s effect on LN229 cell migration. (a) Representative western blots of JNK and p38 phosphorylation at 3 h after scratch in PL-treated LN229 cells. GAPDH was used as a loading control. (b) Statistical analysis of p38 and JNK phosphorylation of western blot results. Normal, unscratched cultures without PL treatment. compared to group of 0 μM of PL; compared to group of 20 μM of PL treatment alone. (c) Representative micrographs showing the effects of PL on LN229 cell migration at 6 h after scratch in the presence of PL, SB203580, or SP600125. SB203580 (10 μM) and SP600125 (10 μM) were administrated 2 h before PL treatment. Bar: 50 μm. (d) Statistical analysis of migrated distance of LN229 cells upon PL, SB203580, or SP600125. .
