Piperlongumine Inhibits Migration of Glioblastoma Cells via Activation of ROS-Dependent p38 and JNK Signaling Pathways
Figure 5
Effects of PL on LN229 cell migration in transwell cell migration assay. (a) Representative micrographs show the effects of PL on LN229 cell migration and the cell morphology of migrated LN229 cells. LN229 cells were seeded into the upper chamber of transwell apparatus in DMEM containing PL (0, 5, or 10 μM) together with or without NAC (3 mM), SB203580 (10 μM), or SP600125 (10 μM). The medium in the lower chamber was replaced with DMEM containing 10% FBS in order to induce cell migration 6 h after PL incubation. Migrated cells in the lower surface of the filter were stained and microphotographed 24 h after cell migration induction by serum. (b) Statistical analysis of migrated cell numbers in different groups. compared to group of 0 μM of PL (DMSO); compared to group of 10 μM of PL treatment alone. (c) Morphology of migrated LN229 cells at higher magnification.
