Mitochondria-Targeted Antioxidant Prevents Cardiac Dysfunction Induced by Tafazzin Gene Knockdown in Cardiac Myocytes
Figure 6
Mito-Tempo normalized contractile dysfunction induced by cardiolipin deficiency due to tafazzin knockdown. Cardiac myocytes isolated from adult mouse hearts were transduced with tafazzin shRNA adenovirus and treated with mito-Tempo overnight. (a) Tafazzin protein. Adenovirus containing tafazzin shRNA efficiently knocked down tafazzin protein. Data represent mean ± SE of 3 separate experiments. versus SCR. (b) Sarcomere shortening. Data represent mean ± SE of 4 separate experiments. versus SCR treated with vehicle and versus TazD treated with vehicle. (c) Sarcomere length. Data represent mean ± SE of 4 separate experiments.
