Antiapoptotic Actions of Methyl Gallate on Neonatal Rat Cardiac Myocytes Exposed to H₂O₂

Figure 3

Methyl gallate protects cell viability by preventing apoptosis. Neonatal RCMs, pretreated with 5 mM NAC or 50 μM MG prior to stress induction with H₂O₂ for 24 hours, were stained with Annexin V and PI and analyzed by flow cytometry to assess apoptotic events by scatter distribution (a). The data is graphically represented asunstained Annexin⁻/PI⁻ (healthy) RCMs (b) and late apoptotic Annexin⁺/PI⁺ cells (c). Stressed cells versus unstressed controls (^††𝑃<0.01) and versus stressed cells pretreated with NAC or MG (**𝑃<0.01, *𝑃<0.05). Data is expressed as means ± S.D, 𝑛=3.

(a)

(b)

(c)