Antiapoptotic Actions of Methyl Gallate on Neonatal Rat Cardiac Myocytes Exposed to H₂O₂

Figure 4

Methyl Gallate prevents DNA damage and inhibits caspase-9 activation. Total DNA was isolated from treated neonatal RCMs as described in methods. The lanes were regrouped from different parts of a representative gel (a) showing: 1Kb + ladder, Lane 1: Control, Lane 2: NAC, Lane 3: MG, Lane 4: H₂O₂, Lane 5: NAC + H₂O₂, and Lane 6: MG + H₂O₂. Fluorescent microscopy images of RCMs stained for cleaved caspase-9 and DAPI (b) showing: Panel 1: Control, Panel 2: MG, Panel 3: H₂O₂, and Panel 4: MG + H₂O₂.

(a)

(b)