Effect of Staurosporine in the Morphology and Viability of Cerebellar Astrocytes: Role of Reactive Oxygen Species and NADPH Oxidase
Figure 2
Reactive oxygen species are involved in cerebellar astrocytes death induced by St. (a) The levels of ROS were measured during different times by the oxidation of dihydroethidium (1 μM) as detailed in Section 2. Data are presented as mean ± SEM of four independent experiments. All points are significantly different from control (0 h) (). (b) Representative micrographs of cerebellar astrocytes treated with St (0.5 μM) for 24 in the presence or absence of MnTMPyP (50 μM) or AEBSF (50 μM). Cells were marked with calcein (green) and propidium iodide (red). Scale bar represents 50 μm. (c) Cell viability was determined by the percentage of calcein-positive cells from the total number of cells (calcein-positive cells plus propidium iodide-positive cells). Data are presented as mean ± SEM of three independent experiments.
