Curcumin Inhibits Mitochondrial Injury and Apoptosis from the Early Stage in EAE Mice
Figure 3
(a) Colocalization with an anti-oligodendrocytes antibody (CNPase) and TUNEL (+) cells by double-labeled immunofluorescence in the spinal cord 3 days p.i. (A) TUNEL (+) cells (green), (B) DAPI stain showing the nucleus (blue), (C) anti-oligodendrocyte antibody (CNPase) (red), and (D) merged image of (A)–(C). Arrows indicate TUNEL (+) cells (A), CNPase (+) cells (C), or the colocalized cells (D). Scale bar = 100 m. (b) Colocalization with anti-neuron antibody (MAP-2) and TUNEL (+) cells by double-labeled immunofluorescence in the spinal cord 3 days p.i. (A) TUNEL (+) cells (green), (B) DAPI stain showing the nucleus (blue), (C) anti-MAP-2 antibody (red), and (D) merged image of (A)–(C). Arrows indicate TUNEL (+) cells (A), MAP-2 (+) cells (C), or the colocalized cells (D). Scale bar = 100 m.
