Nrf2 Deficiency Exaggerates Doxorubicin-Induced Cardiotoxicity and Cardiac Dysfunction
Figure 1
Doxorubicin- (Dox-) induced oxidative stress and necrosis in the heart. Male 10–12 wk old WT mice (C57BL/6J) were subjected to single intraperitoneal injection of 25 mg/kg Dox or vehicle (Veh) control, and then the hearts ( for each group at indicated time point) were harvested for immunochemical staining and Western blot analysis. (a) Representative images of 8-OHdG and Evans blue labelling. Left panel: brown staining indicates 8-OHdG. Right panel: green indicates cardiomyocyte membrane. Red indicates necrotic myocardium. (b) Representative HE staining of left ventricle tissue sections from the mice 4 days after treatment. (c) Representative immunoblots of caspase-3 and cleaved caspase-3.
