Nrf2 Deficiency Exaggerates Doxorubicin-Induced Cardiotoxicity and Cardiac Dysfunction

Table 1

Time-course study of echocardiography in WT and Nrf2^−/− mice after Dox treatment.

	WT		Nrf2^−/−
	(𝑛) Vehicle (3)	Dox (5)	Vehicle (3)	Dox (5)
Day 0
LVPW; d (mm)	0.69±0.02	0.65±0.05	0.67±0.04	0.68±0.03
LVPW; s (mm)	1.05±0.02	1.05±0.05	0.98±0.04	0.98±0.03
FS (%)	28.90±1.56	28.31±3.97	29.50±2.00	28.53±1.19
LV Vol; d (µL)	60.41±0.02	60.86±0.01	61.56±0.07	60.53±0.02
LV Vol; s (µL)	23.68±0.04	23.78±0.01	24.08±0.07	23.81±0.03
HR	469.27±52.42	423.62±29.97	438.00±38.73	426.70±50.89
Day 1
LVPW; d (mm)	0.69±0.03	0.68±0.02	0.66±0.01	0.65±0.03
LVPW; s (mm)	1.07±0.03	1.01±0.02	0.99±0.01	0.90±0.03^B;C
FS (%)	31.12±2.90	28.23±1.10	31.03±2.00	20.98±1.94^B;C
LV Vol; d (µL)	61.78±0.09	61.92±0.03	63.02±0.01	60.45±0.02
LV Vol; s (µL)	24.40±0.08	24.10±0.03	24.92±0.07	22.91±0.03
HR	442.06±16.11	429.90±20.29	439.22±11.07	431.73±32.97
Day 2
LVPW; d (mm)	0.71±0.09	0.67±0.05	0.69±0.06	0.60±0.02
LVPW; s (mm)	1.07±0.09	0.97±0.05^A	0.97±0.06	0.83±0.02^B;C
FS (%)	28.03±1.05	23.00±3.37^A	30.29±6.02	17.89±3.16^B;C
LV Vol; d (µL)	62.47±0.04	60.82±0.01	60.92±0.02	58.73±0.07^B;C
LV Vol; s (µL)	25.80±0.03	24.01±0.02	24.35±0.02	20.99±0.02^B;C
HR	451.67±26.50	445.38±20.20	452.44±28.66	444.73±20.33
Day 4
LVPW; d (mm)	0.70±0.06	0.67±0.02	0.66±0.01	0.59±0.03^B;C
LVPW; s (mm)	1.08±0.06	0.96±0.02^A	0.97±0.01	0.79±0.03^B;C
FS (%)	28.60±0.80	21.71±3.40^A	29.13±1.40	18.49±1.36^B;C
LV Vol; d (µL)	63.17±0.04	58.88±0.08^A	60.39±0.03	57.51±0.03^B;C
LV Vol; s (µL)	24.28±0.01	20.21±0.01^A	22.96±0.02	19.34±0.08^B;C
HR	444.06±13.36	437.33±29.66	443.89±19.76	427.60±29.83

Male 10–12 wk old littermates of WT and Nrf2^−/− mice were treated with single intraperitoneal injection of 25 mg/kg Dox or vehicle (Veh) control for 4 days and cardiac function was monitored daily as indicated. LVPW; d: left ventricular posterior wall thickness diastolic; LVPW; s: left ventricular posterior wall thickness systolic; FS: fractional shortening; LV Vol; d: left ventricular volume; diastolic; LV Vol; s: left ventricular volume; systolic; HR: heart rate. Animal number for each group is indicated in the parentheses. ^A𝑃<0.05 versus WT Vehicle; ^B𝑃<0.05 versus Nrf2^−/− Vehicle; ^C𝑃<0.05 versus WT DOX.