The Effect of tert-Butyl Hydroperoxide-Induced Oxidative Stress on Lean and Steatotic Rat Hepatocytes In Vitro

Figure 2

(a) LDH activity (IU/L) in medium of lean (NH) and steatotic rat hepatocytes (SH) in primary culture treated with 5 𝜇M TFP (NH + TFP; SH + TFP), with 0.25 mM tBHP (NH + tBHP; SH + tBHP) or with 5 𝜇M TFP and 0.25 mM tBHP (NH + TFP + tBHP; SH + TFP + tBHP). Cells were exposed to tBHP for a period of 30 min. The values are means ± SD (𝑛=6). ^∗∗∗𝑃<0.001 versus control NH; ^###𝑃<0.001 versus control SH; ^xx𝑃<0.01 and ^xxx𝑃<0.001 versus corresponding group in lean hepatocytes; ^$$$𝑃<0.001 versus SH + tBHP. (b) WST-1 test of nonsteatotic (NH) and steatotic rat hepatocytes (SH) in primary culture treated with 5 𝜇M TFP (NH + TFP; SH + TFP), with 0.25 mM tBHP (NH + tBHP; SH + tBHP), or with 5 𝜇M TFP and 0.25 mM tBHP (NH + TFP + tBHP; SH + TFP + tBHP). Cells were exposed to tBHP for 30 min. The values are means ± SD (𝑛=6). Results are expressed in percent where 100% is the activity of cellular dehydrogenases in control NH. ^###𝑃<0.001 versus control SH; ^xx𝑃<0.01 and ^xxx𝑃<0.001 versus corresponding group in lean hepatocytes. (c) Concentration of albumin (𝜇g/L) in culture medium of nonsteatotic (NH) and steatotic rat hepatocytes (SH) treated with 5 𝜇M TFP (NH + TFP; SH + TFP), with 0.25/0.375 mM tBHP (NH + tBHP; SH + tBHP), or with 5 𝜇M TFP and 0.25/0.375 mM tBHP (NH + TFP + tBHP; SH + TFP + tBHP). Cells were exposed to tBHP for 30 min. The values are means ± SD (𝑛=5). ^∗𝑃<0.05 and ^∗∗∗𝑃<0.001 versus control NH; ^&&&𝑃<0.001 versus NH + tBHP0.25; ^##𝑃<0.01 and ^###𝑃<0.001 versus control SH; ^xxx𝑃<0.001 versus corresponding group in lean hepatocytes.

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