Bach1 Deficiency and Accompanying Overexpression of Heme Oxygenase-1 Do Not Influence Aging or Tumorigenesis in Mice

Figure 2

Effects of Bach1 deficiency in HSCs. (a) The levels of oxidized DCFDA in HSCs in young and aged, wild-type and Bach1-deficient mice. A representative result from three independent experiments is shown. (b) The averages of median of the experiments in the three experiments of (a). The results are expressed as mean ± S.D (𝑛=3); relative values to young wild-type HSCs in each experiment. (c) The numbers of HSCs in young and aged, wild-type and Bach1-deficient mice. The results are expressed as mean ± S.D (𝑛=3). ^∗𝑃<0.05. (d) Transplantations of bone marrow LT-HSCs between wild-type and Bach1-deficient mice. The chimerism data from five wild-type and four Bach1-deficient mice are presented as means ± S.E.M. The chimerism was calculated by a formula, CD45.2 × 100/(CD45.1+CD45.2) (%).

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