Table of Contents Author Guidelines Submit a Manuscript
Oxidative Medicine and Cellular Longevity
Volume 2014 (2014), Article ID 760694, 13 pages
http://dx.doi.org/10.1155/2014/760694
Research Article

Analysis of Oxidative Stress Enzymes and Structural and Functional Proteins on Human Aortic Tissue from Different Aortopathies

1Immunology Department, National Institute of Cardiology “Ignacio Chavez”, Juan Badiano 1, Sección XVI, Tlalpan, 14080 Mexico City, DF, Mexico
2Pathology Department, National Institute of Cardiology “Ignacio Chavez”, Juan Badiano 1, Sección XVI, Tlalpan, 14080 Mexico City, DF, Mexico
3Physiology Department, National Institute of Cardiology “Ignacio Chavez”, Juan Badiano 1, Sección XVI, Tlalpan, 14080 Mexico City, DF, Mexico
4Cardiovascular Surgery Department, National Institute of Cardiology “Ignacio Chavez”, Juan Badiano 1, Sección XVI, Tlalpan, 14080 Mexico City, DF, Mexico

Received 4 April 2014; Revised 28 May 2014; Accepted 28 May 2014; Published 1 July 2014

Academic Editor: Kota V. Ramana

Copyright © 2014 María Elena Soto et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The role of oxidative stress in different aortopathies is evaluated. Thirty-two tissue samples from 18 men and 14 women were divided into: 4 control (C) subjects, 11 patients with systemic arterial hypertension (SAH), 4 with variants of Marfan’s syndrome (MV), 9 with Marfan’s syndrome (M), 2 with Turner’s syndrome, and 2 with Takayasu’s arteritis (TA). Aorta fragments were homogenized. Lipoperoxidation (LPO), copper-zinc and manganese superoxide dismutase (Mn and Cu-Zn-SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), endothelial nitric oxide synthase (eNOS), nitrates and nitrites (/), and type IV collagen, and laminin were evaluated. There was an increase in Mn- and Cu-Zn-SOD activity in SAH, MV, M, and Turner’s syndrome. There was also an increase in CAT activity in M and Turner’ syndrome. GPx and GST activity decreased and LPO increased in all groups. eNOS was decreased in SAH, MV, and M and / were increased in SAH and TA. Type IV collagen was decreased in Turner’s syndrome and TA. Laminin -1 was decreased in MV and increased in M. In conclusion, similarities and differences in oxidative stress in the different aortopathies studied including pathologies with aneurysms were found with alterations in SOD, CAT, GPx, GST, and eNOS activity that modify subendothelial basement membrane proteins.