Endogenous Ceramide Contributes to the Transcytosis of oxLDL across Endothelial Cells and Promotes Its Subendothelial Retention in Vascular Wall

Figure 1

The effects of various inhibitors on ceramide concentration in HUVECs. HUVECs were incubated with 30 μM MYR, 10 μM DES, 30 μM D609, or 10 μM NOE for 12 h. Representative fluorescence microscopic images of ceramide (a) and quantification analysis (b) of immunofluorescent staining in HUVECs. Scale bars are equal to 50 μm. “Negative control” group was processed in the absence of primary antibody. (c) Concentration of ceramides in HUVECs quantified by HPLC-MS analysis. ^∗𝑃<0.05 versus control, 𝑛=3.

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