Research Article

Electrochemically Reduced Water Protects Neural Cells from Oxidative Damage

Figure 2

Protective effect of ERW against H2O2-induced cytotoxicity on N1E115 cells. N1E115 cells were preincubated with FBS/DMEM medium and were treated with serum-free medium made of MQ-water, ERW, L-NAC (0.1, 1 mM), AsA (0.1, 1 mM), or 2 mM NaOH supplemented with or without 200 μM H2O2 for 24 h. A WST-8 kit was used to measure viable cell numbers at 450 nm using a microtiter plate reader. Data are expressed as mean ± standard deviation (SD) for three independent experiments. , . Abs. cont.: absolute control, Pos. cont.: positive control, Auto. ERW: autoclaved ERW.