Anandamide Protects HT22 Cells Exposed to Hydrogen Peroxide by Inhibiting CB1 Receptor-Mediated Type 2 NADPH Oxidase

Figure 5

AEA ameliorated the morphology of HT22 cells exposed to H₂O₂ via CB1. (a) Control cells cultured in drug-free medium. (b) Cells exposed to 10 μM AEA for 3 h. (c) Cells exposed to 200 μM H₂O₂ for 3 h. (d) Cells exposed to 10 μM AEA plus 200 μM H₂O₂ for 3 h. (e) Cells exposed to 10 μM AEA plus 10 μM AM251 at the presence of 200 μM H₂O₂ for 3 h. (f) Cells exposed to CB1 antagonist AM251 of 10 μM plus 200 μM H₂O₂ for 3 h. H₂O₂ markedly damaged the cell morphology and hindered the growth of neurites. AEA attenuated the H₂O₂-induced injury of HT22 cells whereas CB1 antagonist AM251 reversed the AEA-induced protective effect on cell morphology. Bar = 50 μm.

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