Toona sinensis Inhibits LPS-Induced Inflammation and Migration in Vascular Smooth Muscle Cells via Suppression of Reactive Oxygen Species and NF-B Signaling Pathway
Figure 6
Inhibitory effects of TS and GA on LPS-induced MMP activity and expression in A7r5 cells. Cells were preincubated with or without TS (25–75 μg/mL) or GA (5 μg/mL) for 2 h and then stimulated with LPS (100 ng/mL) for 24 h for gelatin zymography and 12 h for western blotting. (a) Cell culture media was then subjected to gelatin zymography to analyze the activities of MMP-2, MMP-9, and t-PA. The activities of these proteins were subsequently quantified by densitometric analysis. (b) Western blotting was performed to analyze MMP-2 and MMP-9 protein levels. Proteins (50 μg) from each sample were resolved by 8%–10% SDS-PAGE. β-Actin was used as an internal control. Relative changes in the protein bands were measured using AlpaEaseFc 4.0 software. Densitometric analysis, with the control being 100%, is shown below the gel data. The results represent the mean ± SD of three assays. significant difference () in comparison to control or LPS-treated groups, respectively.
