Piperlongumine Induces Apoptosis and Synergizes with Cisplatin or Paclitaxel in Human Ovarian Cancer Cells

Figure 5

ROS generation was critical for PL-induced apoptosis in OVCAR3 ovarian cancer cells. (a) ROS accumulation and (c) cell apoptosis in PL-treated OVCAR3 cells were reversed by NAC. Cells were treated with PL (10 μM) for 24 hr in the presence or absence of 3 mM NAC pretreatment for 1 hr. The apoptosis was detected by FCM with Annexin V/PI staining and the expression of C-PARP proteins was analyzed by Western blot. (c) The quantified results of (d). (b) Representative Western blotting analysis of C-PARP in OVCAR3 cells treated as indicated. β-actin was used as loading control. ^∗𝑃<0.05 and ^∗∗𝑃<0.01 versus corresponding control.

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