Effects of Downregulation of MicroRNA-181a on H₂O₂-Induced H9c2 Cell Apoptosis via the Mitochondrial Apoptotic Pathway

Figure 5

Effects of the miR-181a and anti-miR-181a on H₂O₂-induced H9c2 cell ROS generation. (a) The intracellular ROS levels were estimated using the probe DCFH-DA. ROS production was observed using a fluorescence microscope. (b) The ROS production was quantified by a fluorescence microplate reader, with the fluorescence read at 485 nm for excitation and 530 nm for emission. The cellular fluorescence intensities were expressed as the multiple of the level in the control group. ^∗𝑃<0.01 versus control (CTL); ^&𝑃<0.01 versus miR-CTL; ^#𝑃<0.01 versus anti-CTL group; the values represent the mean ± SEM; 𝑛=5.

(a)

(b)