Effects of Downregulation of MicroRNA-181a on H2O2-Induced H9c2 Cell Apoptosis via the Mitochondrial Apoptotic Pathway
Figure 7
Effects of the miR-181a and anti-miR-181a on the H2O2-induced reduction of the mitochondrial membrane potential. (a) The mitochondrial membrane potential was assessed by the lipophilic cationic probe JC-1. An orange signal indicates the aggregation of JC-1 in the mitochondria. A green signal represents cytosolic JC-1 monomers, indicative of the loss of the mitochondrial membrane potential. Merged images show the colocalization of the JC-1 aggregates and monomers. Scale bar: 25 μm. (b) Quantitative analysis of the membrane potential in (a). The of the H9c2 cardiomyocytes in each group was calculated as the ratio of orange to green fluorescence, expressed as the multiple of the level in the control group. versus control (CTL); versus miR-CTL; versus anti-CTL group; the values represent the mean SEM, .
