Effects of Downregulation of MicroRNA-181a on H2O2-Induced H9c2 Cell Apoptosis via the Mitochondrial Apoptotic Pathway
Figure 8
Effects of the miR-181a and anti-miR-181a on H2O2-induced cytochrome c release from the mitochondria in H9c2 cells. Fluorescence microscopy analysis of H9c2 cells transfected with miRNAs for 6 h prior to exposure to H2O2 (400 μM) for 2 h shows immunostaining of cytochrome c (green), Mito tracker staining of the mitochondria (red) and merged images of the two, showing colocalization in yellow. Upon the release of cytochrome c from the mitochondria, green fluorescence can be seen independently. Scale bar: 25 μm. The images presented are representative of three independent experiments.
