Effects of Downregulation of MicroRNA-181a on H₂O₂-Induced H9c2 Cell Apoptosis via the Mitochondrial Apoptotic Pathway

Table 2

Sequences of the primers used in the SYBR-green-based quantitative RT-PCR validation.

Primer name	Primer sequence	Tm (°C)	Length (bp)
U6	F: 5′GCTTCGGCAGCACATATACTAAAAT3′	60	89
	R: 5′CGCTTCACGAATTTGCGTGTCAT3′
rno-miR-423	F: 5′TAAGCTCGGTCTGAGGC3′	60	65
	R: 5′CAGTGCGTGTCGTGGA3′
rno-miR-7a	F: 5′GGGGTGGAAGACTAGTGATT3′	60	67
	R: 5′CAGTGCGTGTCGTGGA3′
rno-miR-181a	F: 5′GGGCAGCCTTAAGAGGA3′	60	64
	R: 5′CAGTGCGTGTCGTGGA3′
rno-miR-125a	F: 5′GCTCCCTGTAGACCCTTTA3′	60	67
	R: 5′CAGTGCGTGTCGTGGAGT3′