Novel Functional Changes during Podocyte Differentiation: Increase of Oxidative Resistance and H-Ferritin Expression

Figure 5

TBARS levels are elevated in nondifferentiated podocytes but not in differentiated cells under stress conditions. (a) Nondifferentiated and differentiated podocytes were exposed to H₂O₂ (0–500 μmol/L in HBSS) for 4 hours, followed by measurement of TBARS levels. (b) Nondifferentiated and differentiated podocytes were subjected to heme treatment (0–5 μmol/L in HBSS) for 4 hours, followed by measurement of TBARS levels. Results are expressed as fold increase over vehicle-treated controls and shown as mean values ± SEM of three independent experiments each performed in triplicates. ^∗𝑃<0.05, ^∗∗𝑃<0.01, and ^∗∗∗𝑃<0.001.

(a)

(b)