Oxidative Medicine and Cellular Longevity / 2014 / Article / Fig 5

Research Article

Novel Functional Changes during Podocyte Differentiation: Increase of Oxidative Resistance and H-Ferritin Expression

Figure 5

TBARS levels are elevated in nondifferentiated podocytes but not in differentiated cells under stress conditions. (a) Nondifferentiated and differentiated podocytes were exposed to H2O2 (0–500 μmol/L in HBSS) for 4 hours, followed by measurement of TBARS levels. (b) Nondifferentiated and differentiated podocytes were subjected to heme treatment (0–5 μmol/L in HBSS) for 4 hours, followed by measurement of TBARS levels. Results are expressed as fold increase over vehicle-treated controls and shown as mean values ± SEM of three independent experiments each performed in triplicates. , , and .
976394.fig.005a
(a)
976394.fig.005b
(b)

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