Panaxatriol Saponins Attenuated Oxygen-Glucose Deprivation Injury in PC12 Cells via Activation of PI3K/Akt and Nrf2 Signaling Pathway
Figure 4
Effect of PTS on Nrf2 nuclear translocation in PC12 cells. (a) Determination of Nrf2 in the cell nucleus. PC12 cells were treated with PTS at fixed concentration (16 μg·mL−1) for different time points as indicated. The nuclear proteins were isolated as described in Section 2. Nrf2 and nuclear marker lamin b1 were detected by Western blotting using specific antibodies. The blots were representative of three independent experiments. (b) Immunohistochemical determination of Nrf2 nuclear translocation. Cells were treated with 16 μg·mL−1 for 6 h; then Nrf2 protein and nuclei were stained as described in Section 2. Three independent experiments were formed and the representative data are shown here. , significantly different from vehicle group. Scale of bar is 50 μm.
