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Oxidative Medicine and Cellular Longevity
Volume 2015, Article ID 154164, 13 pages
http://dx.doi.org/10.1155/2015/154164
Research Article

Impact of Procyanidins from Different Berries on Caspase 8 Activation in Colon Cancer

1Laboratoire d’Innovation Thérapeutique, UMR 7200, Faculté de Pharmacie, Université de Strasbourg, 74 route du Rhin, BP 24, 67401 Illkirch Cedex, France
2Merck Millipore Bioscience Division, 78180 Saint-Quentin-en-Yvelines, France
3Department of Medical Chemistry and Biochemistry, Palacký University, Hněvotínská 3, 775 15 Olomouc, Czech Republic
4UMR 7200 CNRS, Faculté de Pharmacie, 75 route du Rhin, 67400 Illkirch, France

Received 31 July 2014; Revised 10 October 2014; Accepted 11 October 2014

Academic Editor: David Vauzour

Copyright © 2015 Carole Minker et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Scope. The aim of this work is to identify which proapoptotic pathway is induced in human colon cancer cell lines, in contact with proanthocyanidins extracted from various berries. Methods and Results. Proanthocyanidins (Pcys) extracted from 11 berry species are monitored for proapoptotic activities on two related human colon cancer cell lines: SW480-TRAIL-sensitive and SW620-TRAIL-resistant. Apoptosis induction is monitored by cell surface phosphatidylserine (PS) detection. Lowbush blueberry extract triggers the strongest activity. When tested on the human monocytic cell line THP-1, blueberry Pcys are less effective for PS externalisation and DNA fragmentation is absent, highlighting a specificity of apoptosis induction in gut cells. In Pcys-treated gut cell lines, caspase 8 (apoptosis extrinsic pathway) but not caspase 9 (apoptosis intrinsic pathway) is activated after 3 hours through P38 phosphorylation (90 min), emphasizing the potency of lowbush blueberry Pcys to eradicate gut TRAIL-resistant cancer cells. Conclusion. We highlight here that berries Pcys, especially lowbush blueberry Pcys, are of putative interest for nutritional chemoprevention of colorectal cancer in view of their apoptosis induction in a human colorectal cancer cell lines.