Impact of Procyanidins from Different Berries on Caspase 8 Activation in Colon Cancer

Figure 5

Effect of lowbush blueberry proanthocyanidins (Pcys) (50 μg/mL) on the density of death receptors present at the cell surface of SW620 (a) and SW480 (b) cells. TRAIL-R1 receptor is specifically recognized by a monoclonal antibody Ac1 coupled to Alexa 488 (which emits at 525 nm) whereas TRAIL-R2 receptor is specifically recognized by a monoclonal antibody Ac2 coupled to phycoerythrin (which emits at 575 nm). Fas receptor is specifically recognized by a monoclonal antibody Ac3 coupled to phycoerythrin-Cy5 (PE-Cy5) (which emits at 670 nm). TRAIL-R1 receptor presence is materialized by the mean green fluorescence emitted by Ac1 antibody on SW620 or SW480 cell lines, while TRAIL-R2 receptor is materialized by the mean yellow fluorescence emitted by Ac2 antibody and Fas receptor is materialized by the red green fluorescence emitted by Ac3 antibody. Statistical significant differences ( independent experiments) based on mean fluorescence (AU) of the cell population between labeled cells in absence of proanthocyanidins and cells labeled and treated with Pcys are represented by the symbol “.”

(a)

(b)