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Oxidative Medicine and Cellular Longevity
Volume 2015 (2015), Article ID 160568, 7 pages
http://dx.doi.org/10.1155/2015/160568
Research Article

CO2 Pneumoperitoneum Preserves β-Arrestin 2 Content and Reduces High Mobility Group Box-1 (HMGB-1) Expression in an Animal Model of Peritonitis

1Department of Pediatric, Gynecological, Microbiological and Biomedical Sciences, University of Messina, Azienda Ospedaliera Universitaria Policlinico “G. Martino”, Via Consolare Valeria 1, 98125 Messina, Italy
2Department of Clinical and Experimental Medicine, University of Messina, Azienda Ospedaliera Universitaria Policlinico “G. Martino”, Via Consolare Valeria 1, 98125 Messina, Italy
3Department of Public Health, University of Messina, Azienda Ospedaliera Universitaria Policlinico “G. Martino”, Via Consolare Valeria 1, 98125 Messina, Italy

Received 10 October 2014; Revised 11 January 2015; Accepted 26 January 2015

Academic Editor: Angel Catalá

Copyright © 2015 Angela Simona Montalto et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Laparoscopy (LS) has been shown to decrease the inflammatory sequelae of endotoxemia. β-arrestin 2 plays an important function in signal transduction pathway of TLR4. High mobility group box-1 (HMGB-1) is involved in the delayed systemic inflammatory response. We investigated the effects of CO2 insufflation on liver, lung, and kidney expression of both β-arrestin 2 and HMGB-1 during sepsis. Cecal ligation and puncture (CLP) was performed in male rats and 6 h later the animals were randomly assigned to receive a CO2 pneumoperitoneum or laparotomy. Animals were euthanized; liver, lung, and kidney were removed for the evaluation of β-arrestin 2 and HMGB-1 expression. Immunohistochemical detection of myeloperoxidase (MPO) was investigated in lung and liver and bacterial load was determined in the peritoneal fluid. CO2 pneumoperitoneum reduced peritoneal bacterial load, increased the expression of β-arrestin 2, and blunted the expression of the potent proinflammatory HMGB-1 in liver, lung, and kidney compared with laparotomy. Liver and lung MPO was markedly reduced in rats subjected to LS compared with laparotomy. We believe that CO2 exerts an early protective effect by reducing bacterial load and likely toll-like receptor activation which in turn leads to a preserved β-arrestin 2 expression and a reduced HMGB-1 expression.