Research Article
Copper Uptake in Mammary Epithelial Cells Activates Cyclins and Triggers Antioxidant Response
Figure 2
The uptake of Cu during the time, as determined by solid sampling-graphite furnace atomic absorption spectroscopy. (a) Concentration of Cu in whole cells of MCF10A treated with 50 μM CuSO4 and in untreated cells, during a period of 48 hours. When compared to controls, cells incubated with 50 μM of CuSO4 showed higher Cu(II) levels after 4, 24, and 48 hours. (b) The purities of the nuclei extracted from nontumor MCF10A cells were verified by Western blot analyses. Rabbit antihistone H3 N-terminal and rabbit anti-human Cu/Zn-superoxide dismutase 1 polyclonal antibodies were used to detect the corresponding proteins in the cytosolic and nuclear fractions. (c) Concentration of Cu in the nuclei of controls and cells treated with 50 μM CuSO4 after 48 hours. Significant differences between Cu-treated and untreated cells are indicated by asterisks (, ).
| (a) |
| (b) |
| (c) |