Propofol Attenuates Small Intestinal Ischemia Reperfusion Injury through Inhibiting NADPH Oxidase Mediated Mast Cell Activation

Figure 1

Effects of LPS, Compound 48/80, and hydrogen peroxide (H₂O₂) on the degranulation of RBL-2H3 cells with or without NAC or propofol treatment. (a) β-Hexosaminidase release after LPS treatment for 1 h or 4 h, (b) β-hexosaminidase release after Compound 48/80 stimulation for 1 h, and (c) β-hexosaminidase release after H₂O₂ treatment for 1 h. (d) and (e) quantified the degranulation of RBL-2H3 cells, pretreated with NAC or propofol for 12 h, induced by H₂O₂ stimulation for 1 h, respectively. (f) Cell viability of IEC-6 cells treated with tryptase for 12 h. Results were expressed as percentage of control group; error bars represented the standard error of the mean. (g) Lactate dehydrogenase (LDH) activity of IEC-6 cells treated with tryptase for 2 h. Results were expressed as Mean ± SEM. versus control group, versus H₂O₂ 500 μM group, versus propofol 0.5 μg/mL group.

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