Enhancement of the Acrolein-Induced Production of Reactive Oxygen Species and Lung Injury by GADD34

Figure 4

Low level of pulmonary inflammation in GADD34-knockout mice induced by acrolein. Lungs were collected from wild-type and GADD34-knockout mice at days 7 and 28 after 5 μmol/kg acrolein instillation. (a) Alveolar macrophages as and (b) neutrophils as Gr-1⁺CD11b⁺ were confirmed by FACS. (c) The expression of phospho-NF-κB p65. (d) The expressions of macrophage type I markers, TNFα, IL-6, and Irf5, and macrophage type II markers, Arg-1, Mrc-2, and Retnla, were analyzed by quantitative real-time PCR. (e) Wild-type and GADD34-knockout mice macrophages were cultured in 12-well plastic plates and stimulated with 10 μM acrolein for 12 and 24 h. Supernatants were taken and IL-6 expression was analyzed by ELISA. Data shown are the mean ratios ± SE of three separate experiments. Data are represented as means ± s.e.m. , .

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