Effects of Docosahexaenoic Supplementation and In Vitro Vitamin C on the Oxidative and Inflammatory Neutrophil Response to Activation

Table 3

Effects of PMA activation, in vitro vitamin C, and DHA diet supplementation on the distribution of catalase (CAT) and myeloperoxidase (MPO) activities between intracellular and extracellular neutrophil compartments.

		Control	PMA	PMA + VitC	S	A	S × A
Extracellular activity
CAT (K/10^9 cells)	Placebo	16.2 ± 1.9	39.4 ± 8.1^#	46.1 ± 7.3^#	X	X
	Experimental	10.3 ± 3.2	112 ± 31^#*	90.6 ± 17.8^#
MPO (nkat/10^9 cells)	Placebo	9.18 ± 0.86	34.3 ± 3.8^#	135 ± 25^#$		X
	Experimental	6.94 ± 1.07	40.3 ± 4.3^#	84.5 ± 33.6^#$
Intracellular activity
CAT (K/10^9 cells)	Placebo	62.1 ± 12.9	28.2 ± 6.0^#	77.2 ± 7.29^$		X
	Experimental	67.2 ± 6.26	21.6 ± 3.0^#	68.8 ± 11.9^$
MPO (nkat/10^9 cells)	Placebo	31.5 ± 11.8^a	38.1 ± 8.93^a	1.19 ± 0.06^c		X	X
	Experimental	16.9 ± 2.4^ac	83.6 ± 18.5^b	1.23 ± 0.06^c

Statistical analysis: two-way ANOVA, . S: supplementation effect; A: activation effect; S × A: interaction between supplementation and activation effects. X indicates significant effect of the statistical factor S, A, or S × A. *Differences between placebo and experimental groups; ^#difference with respect to the control group; ^$differences between PMA and PMA + VitC group. When interaction S × A exists between supplementation and activation factors, different letters reveal significant differences. Results are the mean ± SEM.