Effects of Docosahexaenoic Supplementation and In Vitro Vitamin C on the Oxidative and Inflammatory Neutrophil Response to Activation

Table 5

Effects of PMA activation, in vitro vitamin C, and DHA diet supplementation on inflammatory gene expression.

		Control	PMA	PMA + VitC	ANOVA
					S	A	S × A
Gene expression
COX2	Placebo	1.00 ± 0.12	4.37 ± 1.65^#	1.21 ± 0.31^$		X
	Experimental	1.14 ± 0.25	2.32 ± 0.81	1.54 ± 0.33
NFκβ	Placebo	1.0 ± 0.14^a	1.91 ± 0.34^b	0.88 ± 0.09^a	X	X	X
	Experimental	0.90 ± 0.08^a	0.98 ± 0.14^a	0.84 ± 0.06^a
IL8	Placebo	1.00 ± 0.15ª	3.19 ± 1.19^b	1.01 ± 0.36^a			X
	Experimental	0.89 ± 0.13^a	0.89 ± 0.12^a	1.09 ± 0.18^a
TNFα	Placebo	1.00 ± 0.12	2.30 ± 0.51^#	0.99 ± 0.14		X
	Experimental	1.03 ± 0.15	1.24 ± 0.26	0.97 ± 0.12^$
MPO	Placebo	1.00 ± 0.16	1.32 ± 0.27	1.73 ± 0.59	X	X
	Experimental	2.09 ± 0.61	1.86 ± 0.66	4.72 ± 1.23^#$

Statistical analysis: two-way ANOVA, . S: supplementation effect; A: activation effect; S × A: interaction between supplementation and activation effects. X indicates significant effect of the statistical factor S, A, or S × A. ^#Difference with respect to the control group; ^$differences between PMA and PMA + VitC group. When interaction S × A exists between supplementation and activation factors, different letters reveal significant differences. Results are the mean ± SEM.