Daily Oxygen/O₃ Treatment Reduces Muscular Fatigue and Improves Cardiac Performance in Rats Subjected to Prolonged High Intensity Physical Exercise

Figure 4

Western blotting analysis of Mn-SOD, GPx1, and LDH expression levels in cardiac tissue. (a) shows the cardiac expression levels of Mn-SOD, GPx1, LDH, and β-actin (used as control) in rats subjected to prolonged high intensity exercise and insufflated with oxygen (ET + O₂) and with oxygen/ozone mixture at three different doses (100, 150, and 300 μg/kg). The expression levels of Mn-SOD (b), GPx1 (c), and LDH (d) are reported as densitometric units (-axis). Starting at dose of 150 μg/kg, with the maximum effect at dose of 300 μg/kg, ozone treatment significantly increased the expression level of the antioxidant enzymes Mn-SOD and GPX, while it lowered the expression levels of LDH. Significant differences with respect to ET + O₂ are shown as and . ET = exercise trained rats running on treadmill.

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