Daily Oxygen/O₃ Treatment Reduces Muscular Fatigue and Improves Cardiac Performance in Rats Subjected to Prolonged High Intensity Physical Exercise

Table 2

Physical, hemodynamic, and morphometric parameters recorded after 5 weeks of oxygen/ozone (O₂/O₃) treatment (2–6th week of the protocol) in rats subjected to a prolonged high intensity exercise.

	ET + O2	ET + O2/O3 100	ET + O2/O3 150	ET + O2/O3 300
Physical parameters
Body weight (g)	216 ± 28.5	213.2 ± 22.7	200.7 ± 23.7	188.6 ± 19.1
Heart weight (g)	0.81 ± 0.09	0.75 ± 0.10	0.73 ± 0.11	0.71 ± 0.07
Tibial length (cm)	3.89 ± 0.03	3.93 ± 0.04	3.96 ± 0.04	4 ± 0.05
Heart weight/tibial length (g/cm)	0.21 ± 0.06	0.19 ± 0.07	0.18 ± 0.08	0.18 ± 0.05
Hemodynamic parameters
MAP (mmHg)	129 ± 1.5	128.3 ± 1.7	123 ± 1.4	112 ± 1.1
Heart rate (bpm)	431 ± 6.8	427 ± 7.9	415 ± 6	405 ± 5.5
Morphometric parameters
LV mass (g)	0.45 ± 0.07	0.31 ± 0.08	0.28 ± 0.05	0.23 ± 0.03
RWT	0.041 ± 0.004	0.043 ± 0.004	0.031 ± 0.002	0.026 ± 0.003
Systolic function
LVFS (%)	51 ± 1.4	51.8 ± 1.2	54.6 ± 1.3	57.2 ± 1.2
LVEF %	60.2 ± 1.4	61 ± 1.8	64.2 ± 1.6	66.1 ± 1.5
VCF (circ./sec)	0.0065 ± 0.0005	0.0068 ± 0.0003	0.0075 ± 0.0002	0.0082 ± 0.0003
Diastolic function
IVRT (ms)	31 ± 2.5	33.5 ± 3.1	36.6 ± 1.7	39.5 ± 1.6
ratio (ms)	2.9 ± 0.05	2.9 ± 0.06	3.06 ± 0.04	3.1 ± 0.04
Global index
MPI	0.78 ± 0.04	0.79 ± 0.03	0.88 ± 0.03	0.89 ± 0.02

MAP = mean arterial pressure; LV = left ventricular mass and relative wall thickness (RWT); EF = ejection fraction; FS = fractional shortening and velocity of circumferential fiber shortening (VCF); IVRT = Absolute left ventricular isovolumetric relaxation time; ratio = ratio of maximal early diastolic peak velocity/late peak velocity of mitral flow. MPI = myocardial performance index; ms = milliseconds. Significant differences with respect to ET + O₂ are shown as p < 0.05 and p < 0.01. ET = exercise trained rats running on treadmill.