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Oxidative Medicine and Cellular Longevity
Volume 2015, Article ID 212964, 11 pages
http://dx.doi.org/10.1155/2015/212964
Clinical Study

Serum Oxidative Stress Markers and Genotoxic Profile Induced by Chemotherapy in Patients with Breast Cancer: A Pilot Study

1Laboratory of Genetic Toxicity, Postgraduate Program in Pharmaceutical Sciences, Federal University of Piauí, 64049-550 Teresina, PI, Brazil
2Postgraduate Program in Genetics, State University of São Paulo, 14049-900 Ribeirão Preto, SP, Brazil
3Laboratory of Experimental Cancerology, Department of Biophysics and Physiology, Postgraduate Program in Pharmaceutical Sciences, Federal University of Piauí, 64049-550 Teresina, PI, Brazil
4Chemistry Department, University of Alabama in Huntsville, Huntsville, AL 35899, USA
5Laboratory of Research in Experimental Neurochemistry, Postgraduate Program in Pharmaceutical Sciences, Federal University of Piauí, 64049-550 Teresina, PI, Brazil

Received 29 November 2014; Revised 2 March 2015; Accepted 2 March 2015

Academic Editor: Amit Tyagi

Copyright © 2015 Antonio Luiz Gomes Júnior et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of this study was to evaluate the oxidative parameters of erythrocytes and genotoxicity in leukocytes of patients with breast cancer. Oxidative parameters were detected by spectrophotometry and genotoxic damage by single cell gel electrophoresis. Twenty-eight women with breast cancer were monitored before chemotherapy and after the second and fourth cycles of therapy with cyclophosphamide and doxorubicin. After the fourth cycle, increases () in the reactive substances to thiobarbituric acid levels, nitrite content, and superoxide dismutase activity and high rates of DNA damage in leukocytes were observed when compared with healthy women group and baseline levels. Similarly, after the second cycle, the same parameters were increased () when compared with baseline levels. Increase in catalase activity was detected only after the fourth cycle and reduced glutathione levels and glutathione peroxidase activity were decreased in all cycles when compared with healthy women, as well as after the second and fourth chemotherapy cycles compared to baseline (). Patients with breast cancer presented an indicative of oxidative stress before, during, and after chemotherapy, as well as increased genotoxic damage in all stages of treatment, demonstrating the clinical applicability of this investigation.