Figure 4: DNA damage investigation by the alkaline comet assay (single cell gel electrophoresis) carried out in lymphocytes of patients with breast cancer before (C0), during (C2), and after AC (C4) chemotherapy. Control group (CG) is represented by healthy patients. Analyses were performed by light microscopy at 100x magnification with immersion oil. Values ​​represent mean ± S.E.M. when compared with control group (CG) by ANOVA followed by -Student-Newman-Keuls. when compared with C0 group (before chemotherapy) and when compared with C2 group (second cycle of chemotherapy).