Epigallocatechin-3-Gallate Enhances the Therapeutic Effects of Leptomycin B on Human Lung Cancer A549 Cells

Figure 2

The effects of LMB, LMB + EGCG, or NAC on ROS production. Data are expressed as means ± SE of three replicate experiments. Cells were seeded in 96-well plates (5 × 10³ cells/well) and treated for 30 min, 2, 4, 12, 24, and 48 h. A549 cells were also pretreated for 2 h with 10 mM NAC and then treated with LMB and/or EGCG for 2 and 4 h upon NAC removal. ROS formation in cells was detected using DCFDA and the ROS fluorescence was measured at an excitation of 485 nm and an emission of 535 nm. The fluorescence in treated cells at each time point was compared to the respective control. E: 20 μM EGCG; 0.5L: 0.5 nM LMB; 5L: 5 nM LMB; NAC: 10 mM NAC pretreated for 2 h. as compared to control; ^# as compared to 5 nM LMB.