Inhibition of Adenylyl Cyclase Type 5 Increases Longevity and Healthful Aging through Oxidative Stress Protection

Figure 2

ERK pathway and oxidative stress in aging AC5 KO. (a) Western blotting of MnSOD in the hearts of aging WT and AC5 KO mice. The levels of MnSOD are significantly greater in AC5 KO mice compared to WT mice (^∗). Data are expressed as mean ± SEM. (b) Cell viability was tested in response to oxidative stress in neonatal cardiac myocytes from AC5 KO and WT. Myocardial cells were treated with H₂O₂ (25 μM) and evaluated for cell viability using Cell Titer-Blue Cell Viability Assay. AC5 neonatal myocytes showed resistance to oxidative stress and DNA damage (^∗ versus WT). Data are presented as mean ± SEM. (c) By western blotting, the level of ERK phosphorylation was significantly increased in AC5 KO mice compared with WT (^∗). Data are expressed as mean ± SEM. (d) Signaling diagram for AC5 regulation of oxidative stress through the ERK pathway is shown. Data is redrawn from Yan et al. [4].

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