Physical Training Regulates Mitochondrial Parameters and Neuroinflammatory Mechanisms in an Experimental Model of Parkinson’s Disease

Figure 3

The effects of two physical training protocols on nitric oxide (a and b, ) and NF-κB p65 (c and d, ) levels in the striatum and hippocampus of mice exposed to 6-OHDA. For western blotting, hippocampus and striatum protein extracts containing 0.2 mg total protein were separated by SDS-PAGE, transferred to nitrocellulose membranes, and probed with anti-NF-κB-p65 antibody. Nitrite generation by NO was assayed spectrophotometrically. Values for NF-κB p65 are presented as mean area × intensity of bands ± SEM (arbitrary units), and oxide nitric levels are expressed as mean ± SEM. , Sham versus U + 6-OHDA; , U + 6-OHDA versus training groups plus 6-OHDA. Untrained + vehicle (Sham), strength training + vehicle (STR), treadmill training + vehicle (TTR), untrained + 6-OHDA (U + 6-OHDA), strength training + 6-OHDA (STR + 6-OHDA), and treadmill training + 6-OHDA (TTR + 6-OHDA).

(a) Striatum

(b) Hippocampus

(c) Striatum

(d) Hippocampus