The Effect of Age on Osteogenic and Adipogenic Differentiation Potential of Human Adipose Derived Stromal Stem Cells (hASCs) and the Impact of Stress Factors in the Course of the Differentiation Process

Figure 5

Age-related changes in hASC cultured under osteogenic conditions. Growth curves (a), population doubling time (b). Morphological evaluation after 16 days of culture, magnification ×100, scale bars: 250 μm; SEM pictures; magnification ×2000, scale bars: 20 μm (c). Mean bone nodules number (d), composition of mineral matrix (e), and average nodules diameter (f). Results expressed as mean ± SD. value < 0.05, value < 0.01, and value < 0.001. (>20), (>50), (>60), and (>70): donor age range; d11: day 11th, d14: day 14th, and on: osteo-nodule.

(a)

(b)

(c)

(d)

(e)

(f)