Cardiac and Vascular Synergic Protective Effect of Olea europea L. Leaves and Hibiscus sabdariffa L. Flower Extracts

Figure 2

Cell viability of HUVECs treated with OEE, HSE, and their mixture. HUVECs were treated as described in Methods section. Cell viability was analysed by the MTT test as reported in the Methods section ((a), (b), and (c)). Cell viability was analysed by flow cytometry. Cells were double-labelled with Annexin V-PE 7 AAD and analyzed by a Guava EasyCyte flow cytometer ((d), (e), and (f)). Data are reported as means ± S.D. of four independent experiments.

(a)

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(d)

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(f)