Cardiac and Vascular Synergic Protective Effect of Olea europea L. Leaves and Hibiscus sabdariffa L. Flower Extracts
Effect of OEE, HSE, and their mixture on intracellular ROS production. HUVECs were treated with OEE (a), HSE (b), and their mixture (c) (0.05–100 μg/mL) for 24 h; oxidative damage was then induced exposing the cells to 100 μM H2O2 for 30 min and intracellular ROS were determined using the peroxide-sensitive fluorescent probe DCFH-DA as described in Methods section. Data are expressed as percent of control cells treated with H2O2. Values represent means ± S.D. of four independent experiments. with respect to H2O2-treated cells.
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