Cardiac and Vascular Synergic Protective Effect of Olea europea L. Leaves and Hibiscus sabdariffa L. Flower Extracts

Table 3

Relaxant activity of OEE, HSE, and their mixture on K⁺-depolarized guinea pig smooth muscle.

Extract	Aorta			GPILSM
	Activity^a (M ± SEM)	(mg/mL)	95% conf. lim.	Activity^a (M ± SEM)	(mg/mL)	95% conf. lim.
OEE	90 ± 1.1	5.15	4.68–5.59	90 ± 1.7	0.77	0.34–1.01
HSE	93 ± 1.4	6.63	6.34–6.92	21 ± 0.7^c
Mixture	70 ± 3.6	5.89	5.56–6.25	90 ± 1.2	1.39	1.13–1.54

Percent inhibition of calcium-induced contraction on K⁺-depolarized (80 mM) guinea-pig aortic strips at 10 mg/mL concentration. The 10 mg/mL concentration gave the maximum effect for most compounds, respectively. ^bCalculated from concentration-response curves (Probit analysis by Litchfield and Wilcoxon [46] with ). When the maximum effect was <50%, the IC₅₀ values were not calculated. ^cAt 5 mg/mL concentration.