Toll-Like Receptor 4 Promotes NO Synthesis by Upregulating GCHI Expression under Oxidative Stress Conditions in Sheep Monocytes/Macrophages

Figure 1

Overexpression of TLR4 in G1 transgenic sheep. (a) Transgene-positive sperm from transgenic sheep were detected by PCR. Lanes: (1) TLR4-positive ovine sperm genomic DNA, (2) TLR4-positive ovine ear genomic DNA, (3) expression vector (p3S-LoxP-TLR4), and (4) negative control. (b) Southern blot analysis of the TLR4 transgene insert in transgenic G1 lambs. “+”: TLR4-transgenic G1 lambs, “−”: negative control, and “c”: TLR4-transgenic sheep. TLR4 expression and translation in mononuclear cells from G1 transgenic lambs were detected by real-time quantitative PCR and ELISA; more TLR4 was detected in transgenic lambs (c) and (d). Blood routine (e) and blood biochemistry (f) examinations were carried out on TLR4-transgenic sheep. There were no statistically significant differences between G1 and WT lambs. Tg: TLR4-transgenic G1 lambs, WT: wild-type. The results are expressed as mean ± SE; in Tg versus WT groups.

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