Apigenin Attenuates Atherogenesis through Inducing Macrophage Apoptosis via Inhibition of AKT Ser473 Phosphorylation and Downregulation of Plasminogen Activator Inhibitor-2

Figure 2

Protective effects of oxidized LDL and native LDL on macrophages. Murine peritoneal macrophages were plated at a density of 1 × 10⁴ cells/well in 96 well plate. Then cells were treated with the indicated concentration of apigenin for 48 h after 1 h preincubation with 50 μg/mL OxLDL. Cell viability was measured by MTT assay. (a) Apigenin significantly reduced the viability of MPM in a dose dependent manner. , compared with the control. (b) Apigenin significantly reduced the viability of MPM pretreated with OxLDL in a time dependent manner. , compared with the control. ((c)-(d)) Apigenin significantly increased the apoptosis of MPM. Each value represents the mean ± S.E.M of quadruplicate determinations from three independent experiments.

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