Apigenin Attenuates Atherogenesis through Inducing Macrophage Apoptosis via Inhibition of AKT Ser473 Phosphorylation and Downregulation of Plasminogen Activator Inhibitor-2

Figure 3

Proteomic differences between murine peritoneal macrophages incubated in the absence or presence of apigenin after stimulated by OxLDL. Cells were treated with/without 50 μM apigenin for 48 h after 1 h pretreatment with 50 μg/mL OxLDL. (a) Representative two-dimensional gel electrophoresis (2DE) gels showing the locations of spots with different intensities in control, OxLDL, and apigenin + OxLDL groups are shown. Protein extraction, 2-DE, and gel staining were performed as previously described. (b) Spot 11 was identified as PAI-2 according to the MOWSE score. (c) Representative results of Western blotting that PAI-2 were significantly increased by OxLDL, but decreased by apigenin. -actin was used as a loading control. Results shown are the representative of three independent experiments.

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