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Oxidative Medicine and Cellular Longevity
Volume 2015, Article ID 379538, 12 pages
Research Article

Apigenin Attenuates Atherogenesis through Inducing Macrophage Apoptosis via Inhibition of AKT Ser473 Phosphorylation and Downregulation of Plasminogen Activator Inhibitor-2

1Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, China
2Guangzhou Institute of Cardiovascular Disease, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, China
3The Key Laboratory of Molecular Biology, State Administration of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong 510515, China
4Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
5Center for Cancer and Inflammation Research, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong

Received 23 December 2014; Revised 24 March 2015; Accepted 24 March 2015

Academic Editor: Kota V. Ramana

Copyright © 2015 Ping Zeng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

OxLDL significantly increased survival rate of MPMs. However, no obvious increased cell viability was observed in phorbol 12-myristate-13-acetateprimed THP-1 cells by pretreated with OxLDL (Figure S1). In MPMs, the increasing cell viability induced by OxLDL was not due to the cell proliferation (Figure S2, S3). PAI-2 siRNAs (Figure S4) and lentivirus (Figure S5) containing empty vector, Akt wild type (WT), 473 serine to glycine (S473G), 308 threonine to glycine (T308G), and double mutant of 473 serine and 308 threonine (S473G-T308G) were constructed and used to study the role of PAI-2 and Akt S473 in the apigenin induced apoptosis of OxLDL loaded MPMs.

  1. Supplementary Material