Thiosemicarbazone p-Substituted Acetophenone Derivatives Promote the Loss of Mitochondrial , GSH Depletion, and Death in K562 Cells
Cell death, apoptosis, and necrosis were triggered in K562 cells by TSC compounds in a dose-dependent manner. K562 cells were incubated with 0.01, 0.1, and 1.0 mmol/L of (a) TSC-Cl, (b) TSC-Me, (c) TSC-F, (d) TSC-H, and (e) TSC-NO2 for 6 h. Apoptosis and necrosis were evaluated via flow cytometric analysis of phosphatidylserine externalization (i.e., annexin V binding) and cell membrane integrity (i.e., 7-AAD staining). White columns correspond to the percentage of the viable cell population, light gray columns correspond to early apoptotic cells, gray columns correspond to late apoptotic cells, and black columns correspond to necrotic cells. The insets show the structures of the compounds with the corresponding calculated values.
Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.