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Oxidative Medicine and Cellular Longevity
Volume 2015 (2015), Article ID 412173, 10 pages
http://dx.doi.org/10.1155/2015/412173
Research Article

Impact of Atmospheric Microparticles on the Development of Oxidative Stress in Healthy City/Industrial Seaport Residents

1Vladivostok Branch of the Far Eastern Center of Physiology and Pathology of Respiration, Institute of Medical Climatology and Rehabilitative Treatment, 73g Russkaya Street, Vladivostok 690105, Russia
2Far Eastern Federal University, 8 Sukhanova Street, Vladivostok 690950, Russia
3Far Eastern Center of Physiology and Pathology of Respiration, 22 Kalinina Street, Blagoveshchensk 675000, Russia
4Far Eastern Geological Institute FEB RAS, 159 Prospekt 100-letiya, Vladivostok 690022, Russia
5Department of Toxicology and Forensics, Medical School, University of Crete, Heraklion, 71300 Crete, Greece

Received 4 November 2014; Revised 7 April 2015; Accepted 8 April 2015

Academic Editor: Vladimir Jakovljevic

Copyright © 2015 Kirill Golokhvast et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Atmospheric microsized particles producing reactive oxygen species can pose a serious health risk for city residents. We studied the responses of organisms to microparticles in 255 healthy volunteers living in areas with different levels of microparticle air pollution. We analyzed the distribution of microparticles in snow samples by size and content. ELISA and flow cytometry methods were employed to determine the parameters of the thiol-disulfide metabolism, peroxidation and antioxidant, genotoxicity, and energy state of the leukocytes. We found that, in the park areas, microparticles with a size of 800 μm or more were predominant (96%), while in the industrial areas, they tended to be less than 50 μm (93%), including size 200–300 nm (7%). In the industrial areas, we determined the oxidative modification of proteins (21% compared to the park areas, ) and DNA (12%, ), as well as changes in leukocytes’ energy potential (53%, ). An increase in total antioxidant activity (82%, ) and thiol-disulfide system response (thioredoxin increasing by 33%, ; glutathione, 30%, with stable reductases levels) maintains a balance of peroxidation-antioxidant processes, protecting cellular and subcellular structures from significant oxidative damage.