Glutathione Suppresses Cerebral Infarct Volume and Cell Death after Ischemic Injury: Involvement of FOXO3 Inactivation and Bcl2 Expression

Figure 7

The mRNA level of Bcl2 in hypoxia exposed b.End3 cells. The mRNA level of Bcl2 was measured using RT-PCR. The mRNA level of Bcl2 was decreased in the hypoxia injury group compared to the normal control group. 1 mM GSH increased the mRNA level of Bcl2 in hypoxia injured b.END3 cells. The mRNA level of Bcl2 was increased in the hypoxia injury group compared to the normal control group. 1 mM GSH reduced mRNA level of Bcl2 in hypoxia-injured b.END3 cells. In Akt inhibitor groups, the mRNA level of Bcl2 shows the reduction compared to the no inhibitor treatment group, respectively. The bar graph shows the quantification of Bcl2 mRNA in all groups. GAPDH was used as an internal control. Each experiment included 5 repeats per condition. Data are expressed as mean ± S.E.M. (, ).