Research Article

In Vitro Toxicity of Epigallocatechin Gallate in Rat Liver Mitochondria and Hepatocytes

Table 1

Biochemical parameters of cultured hepatocytes. LDH leakage (% of total LDH activity), WST-1 test (% of respective controls), albumin production (ng/mL), activity of caspases 3, 8, and 9 in cell lysate (% of respective controls), production of TBARS in cell lysate (μmol/L), reactive oxygen species (ROS) formation using DCFDA (% of respective controls), and TNFα levels in culture medium (pg/mL). All values represent the 24-hour interval. *, **, and *** versus medium-treated controls; , 0.01, and 0.001, respectively. n/a = not measured.

EGCG concentration (μmol/L)
051015203050100

LDH leakage
()
16.1 ± 0.916.3 ± 0.624.9 ± 1.7***37.7 ± 1.8***50.2 ± 1.3***55.3 ± 1.4***56.5 ± 0.8***66.7 ± 2.6***
WST-1
()
100.0 ± 1.8103.1 ± 2.288.1 ± 3.0*51.2 ± 12.5***30.4 ± 8.2***10.5 ± 1.7***2.7 ± 0.2***1.5 ± 0.1***
Albumin
()
60.0 ± 4.449.9 ± 8.9**38.5 ± 3.5***31.3 ± 3.2***21.7 ± 3.8***18.5 ± 0.9***11.6 ± 1.5***6.6 ± 1.4***
Caspase 3 ()100 ± 13121 ± 15690 ± 45***1100 ± 77***1212 ± 50***1274 ± 65***1491 ± 44***763 ± 108***
Caspase 8 ()100.0 ± 18.5n/a108.8 ± 15.7n/an/a339.9 ± 34.6***306.2 ± 32.9***n/a
Caspase 9 ()100.0 ± 12.3n/a105.6 ± 7.2n/an/a116.0 ± 7.0*120.0 ± 11.1**n/a
TBARS
()
0.49 ± 0.080.42 ± 0.040.45 ± 0.060.60 ± 0.230.47 ± 0.110.44 ± 0.050.46 ± 0.090.54 ± 0.09
ROS ()100 ± 8.698 ± 8.482 ± 9.5**80 ± 8.5**87 ± 4.696 ± 12.5110 ± 9.0131 ± 11.5***
TNFα ()80 ± 8.719 ± 15.471 ± 17.4130 ± 5.0209 ± 32.8253 ± 23.1*149 ± 21.899 ± 24.9